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Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography.

Authors
  • Minakuchi, H1
  • Nakanishi, K
  • Soga, N
  • Ishizuka, N
  • Tanaka, N
  • 1 Division of Material Chemistry, Faculty of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-01, Japan, and Department of Polymer Science and Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606, Japan. , (Japan)
Type
Published Article
Journal
Analytical chemistry
Publication Date
Oct 01, 1996
Volume
68
Issue
19
Pages
3498–3501
Identifiers
DOI: 10.1021/ac960281m
PMID: 21619283
Source
Medline
License
Unknown

Abstract

Continuous porous silica rods consisting of a mesoporous (pore size, 14 or 25 nm) silica skeleton of ∼1 μm size and through-pores of ∼1.7 μm were prepared and derivatized to C(18) phase by on-column reaction with octadecyldimethyl-(N,N-diethylamino)silane. The C(18) silica rods gave plate heights of 10-20 μm for aromatic hydrocarbons in 80% methanol and 20-40 μm for insulin in acetonitrile-water mixtures in the presence of trifluoroacetic acid. The performance of the silica rods was much better at a high flow rate than that of conventional columns packed with 5 μm C(18) silica particles having 12 and 30 nm pores, especially for high molecular weight species.

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