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Occurrence of lipopolysaccharide alterations among Tn5 mutants of Rhizobium leguminosarum bv. trifolii strain 24.1 with altered colony morphology.

Authors
  • Szyprowska, S
  • Choma, A
  • Glowacka, M
  • Gronek, E
  • Kitlińska, A
  • Rola, M
Type
Published Article
Journal
Acta biochimica Polonica
Publication Date
Jan 01, 1998
Volume
45
Issue
4
Pages
1001–1009
Identifiers
PMID: 10397346
Source
Medline
License
Unknown

Abstract

Transposon mutants of Rhizobium leguminosarum bv. trifolii 24.1 showing less glossy or smaller colonies were screened for properties usually associated with lipopolysaccharide (LPS) defects in R. leguminosarum, i.e. motility, growth rate, tendency to agglutination in liquid media and symbiotic efficiency. Neither any of the above mutants nor the earlier isolated 24.12 strain, defective in LPS, showed all these properties changed simultaneously. According to PAGE/sodium deoxycholate analysis the mutant 24.12 was the only one producing defective lipopolysaccharide. GC-MS analysis revealed in this mutant qualitative changes in composition of its LPS in comparison with LPS isolated from the parent strain. Other Tn5 mutants produced LPSs similar in composition, however the proportion between LPS I and LPS II differed from that in the parent strain.

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