Obtaining, characterization and evaluation of two candidate preparations for probiotics developed with agroindustrial waste

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Obtaining, characterization and evaluation of two candidate preparations for probiotics developed with agroindustrial waste

Authors
  • Revista MVZ Córdoba
  • Miranda-Yuquilema, José E.1
  • Marin-Cárdenas, Alfredo2
  • Sánchez-Macías, Davinia3
  • García-Hernández, Yaneisy4
Type
Published Article
Journal
Revista Mvz Córdoba
Publisher
Universidad de Cordoba
Publication Date
Jan 09, 2018
Accepted Date
Dec 09, 2017
Volume
23
Issue
1
Pages
6487–6499
Identifiers
DOI: 10.21897/rmvz.1243
Source
MyScienceWork
Keywords
License
Green

Abstract

Objective. Obtain, characterize and evaluate two bio-prepares developed from the sugar cane molasses-orange vinasse fermented with yeast and/or lactic acid bacteria. Materials and methods. A completely randomized design was used, with five repeats per treatment. The evaluated treatments were: T1, Lactobacillus acidophilus, Lactobacillus bulgaricus, Streptococcus thermophilus y T2, the previous bacteria plus Saccharomyces cerevisiae and Kluyveromyces fragilis (L-4 UCLV). The previous mentioned microorganisms were inoculated in a substratum compounded by molasses-vinasse and these were incubated at 37°C for 24 hours. To the bioprepares, physiochemical, microbiological and in vitro tests was made to evaluate the probiotic capacity. Results. Both bioprepares presented a dark brown color, sweet and a pH lesser than 4. The bromatological and microbiologic development were higher (p>0.05) in T2. Both bioprepares the viability was higher than 92%. in vitro tests two bioprepares were resistant to an acid pH, bile salts, broad spectrum of microbial activity and inhibitory effect to E. coli, Salmonella spp. and S. aureus. Conclusions. The bioprepares obtained from sugar cane molasses-orange vinasse fermented with yeast and lactic acid bacteria manifested physiochemical and microbiologic properties appropriated to probiotic products. In in vitro tests, their potential was demonstrated as a probiotic.

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