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Obesity-induced elevated palmitic acid promotes inflammation and glucose metabolism disorders through GPRs/NF-κB/KLF7 pathway.

Authors
  • Qiu, Tongtong1
  • Yang, Xin1
  • Wang, Jingzhou1
  • Pan, Chongge1
  • Chu, Xiaolong1
  • Xiong, Jianyu1
  • Xie, Jianxin1
  • Chang, Yongsheng1, 2
  • Wang, Cuizhe3
  • Zhang, Jun4
  • 1 Medical College of Shihezi University, Bei-Er-Lu, Shihezi, 832000, Xinjiang, China. , (China)
  • 2 Department of Physiology and Pathophysiology, Tianjin Medical University, Tianjin, 300000, China. , (China)
  • 3 Medical College of Shihezi University, Bei-Er-Lu, Shihezi, 832000, Xinjiang, China. [email protected] , (China)
  • 4 Medical College of Shihezi University, Bei-Er-Lu, Shihezi, 832000, Xinjiang, China. [email protected] , (China)
Type
Published Article
Journal
Nutrition & diabetes
Publication Date
Apr 20, 2022
Volume
12
Issue
1
Pages
23–23
Identifiers
DOI: 10.1038/s41387-022-00202-6
PMID: 35443706
Source
Medline
Language
English
License
Unknown

Abstract

Our previous results have shown that obesity-induced excessive palmitic acid (PA) can promote the expression of KLF7, which plays a vital role in regulation of inflammation, glucose metabolism. But the exact mechanism of PA up-regulating the expression of KLF7 is not clear yet. This study is intend to explore whether PA promoting KLF7 expression through GPRs/NF-κB signaling pathway, causing inflammation and glucose metabolism disorders. Cells were blocked GPRs/NF-κB under PA stimulation in vitro to demonstrate the molecular mechanism of PA up-regulates KLF7 expression. The regulatory effect of p65 on KLF7 was detected by luciferase reporter gene assay. Blocking GPRs/NF-κB in diet-induced obesity mice to detect the expression of KLF7, inflammatory cytokines and glucose metabolism related factors, clarifying the effects of GPRs/NF-κB on KLF7 in vivo. In 3T3-L1 adipocytes and HepG2 cells, PA could up-regulate the expression of KLF7 by promoting the GPR40/120-NF-κB signaling pathway, leading to inflammation and reduced glucose consumption (p < 0.05 for both). Luciferase reporter gene assay and ChIP assay showed that p65 could transcriptionally up-regulates the expression of KLF7. In high-fat diet (HFD) mice, after intraperitoneal injection of GPR40 or GPR120 blocker, the levels of p-p65 and KLF7 in epididymal white adipose tissue and liver were significantly decreased (p < 0.05 for both). Pharmacological inhibition of p-p65 significantly attenuated KLF7 expression and improved glucose tolerant and insulin sensitive (p < 0.05 for both). Our results indicate that obesity-induced elevated palmitic acid promotes inflammation and glucose metabolism disorders through GPRs/NF-κB/KLF7 signaling pathway. © 2022. The Author(s).

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