Affordable Access

Nylon wool column--a tool for obtaining monokine-rich eluates in the absence of serum.

Authors
  • Dalmau, S R
  • Freitas, C S
Type
Published Article
Journal
Memórias do Instituto Oswaldo Cruz
Publisher
SciELO
Publication Date
Jan 01, 1990
Volume
85
Issue
2
Pages
153–162
Identifiers
PMID: 2087156
Source
Medline
License
Unknown

Abstract

Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants containing high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 micrograms/ml. Indomethacin did not modify the monokine release, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As a result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifying bioactive peptides from crude broths.

Report this publication

Statistics

Seen <100 times