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Nucleotide sequence of valine tRNA mo5UAC from bacillus subtilis.

Authors
  • Murao, K
  • Hasegawa, T
  • Ishikura, H
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jan 22, 1982
Volume
10
Issue
2
Pages
715–718
Identifiers
PMID: 6801627
Source
Medline
License
Unknown

Abstract

A valine tRNA was purified from Bacillus subtilis W168 by a combined use of several column chromatographic systems. The nucleotide sequence was determined to be pG-G-A-G-G-A-U-U-A-G-C-U-C-A-G-C-D-G-G-G-A-G-A-G-C-A-U-C--G-C-C-U-mo5U-A-C-m6-A -A-G-C-A-G-A-G-G-m7G-U-C-G-G-C-G-G-T-psi-C-G-A-G-C-C-C-G-U-C-A-U-C-C-U-C-C-A-C- C-AOH with the main use of of non-labeled tRNA and with the subsidiary use of [32P]-post-labeled sample. This tRNA contains 5-methoxy-uridine (mo5U) at the wobble position of the anticodon. A binding experiment of valyl-tRNA to ribosome revealed that mo5U is recognized by A and G, and fairly well by U.

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