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The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170.

Authors
  • Kato, C
  • Nakano, Y
  • Horikoshi, K
Type
Published Article
Journal
Archives of microbiology
Publication Date
Jan 01, 1989
Volume
151
Issue
2
Pages
91–94
Identifiers
PMID: 2655551
Source
Medline
License
Unknown

Abstract

The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus beta-lactamase III and Bacillus licheniformis penicillinase.

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