Affordable Access

Nucleotide sequence and analysis of the lethal factor gene (lef) from Bacillus anthracis.

Authors
  • Bragg, T S
  • Robertson, D L
Type
Published Article
Journal
Gene
Publisher
Elsevier
Publication Date
Sep 01, 1989
Volume
81
Issue
1
Pages
45–54
Identifiers
PMID: 2509294
Source
Medline
License
Unknown

Abstract

The nucleotide sequence of the Bacillus anthracis lethal factor (LF) gene (lef) has been determined. LF is part of the tripartite protein exotoxin of B. anthracis along with protective antigen (PA) and edema factor (EF). The apparent ATG start codon, which is located immediately upstream from codons which specify the first 16 amino acids (aa) of the mature secreted LF, is preceded by an AAAGGAG sequence, which is its probable ribosome-binding site. This ATG codon begins a continuous 2427-bp open reading frame which encodes the 809-aa LF-precursor protein with an Mr of 93,798. The mature secreted protein (776 aa; Mr 90,237) was preceded by a 33-aa signal peptide which has characteristics in common with leader peptides for other secreted proteins of the Bacillus species. The codon usage of the LF gene reflects its high (70%) A + T content. The N-terminus of LF (first 300 aa) shared extensive homology with the N-terminus of the anthrax EF protein. Since LF and EF each bind PA at the same site, these homologous regions probably represent their common PA-binding domains.

Report this publication

Statistics

Seen <100 times