Affordable Access

deepdyve-link deepdyve-link
Publisher Website

Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin.

Authors
Type
Published Article
Journal
Genes & Development
0890-9369
Publisher
Cold Spring Harbor Laboratory
Publication Date
Volume
27
Issue
24
Pages
2663–2677
Identifiers
DOI: 10.1101/gad.225888.113
PMID: 24352422
Source
Medline
Keywords
License
Unknown

Abstract

Polycomb-repressive complex 2 (PRC2) comprises specific members of the Polycomb group of epigenetic modulators. PRC2 catalyzes methylation of histone H3 at Lys 27 (H3K27me3) through its Enhancer of zeste (Ezh) constituent, of which there are two mammalian homologs: Ezh1 and Ezh2. Several ancillary factors, including Jarid2, modulate PRC2 function, with Jarid2 facilitating its recruitment to target genes. Jarid2, like Ezh2, is present in poorly differentiated and actively dividing cells, while Ezh1 associates with PRC2 in all cells, including resting cells. We found that Jarid2 exhibits nucleosome-binding activity that contributes to PRC2 stimulation. Moreover, such nucleosome-binding activity is exhibited by PRC2 comprising Ezh1 (PRC2-Ezh1), in contrast to PRC2-Ezh2. The presence of Ezh1 helps to maintain PRC2 occupancy on its target genes in myoblasts where Jarid2 is not expressed. Our findings allow us to propose a model in which PRC2-Ezh2 is important for the de novo establishment of H3K27me3 in dividing cells, whereas PRC2-Ezh1 is required for its maintenance in resting cells.

Statistics

Seen <100 times