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Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.

Authors
  • R, Lucena
  • N, Dephoure
  • Sp, Gygi
  • Doug Kellogg
  • Va, Tallada
  • Rr, Daga
  • J, Jimenez
Type
Published Article
Journal
The Journal of Cell Biology
Publisher
The Rockefeller University Press
Volume
209
Issue
3
Pages
387–402
Identifiers
DOI: 10.1083/jcb.201412144
Source
UCSC Cancer biomedical-ucsc
License
Unknown

Abstract

During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain surrounding the mitotic spindle midzone, here named the midzone membrane domain (MMD), is essential for spindle disassembly in Schizosaccharomyces pombe cells. We show that, during anaphase B, Imp1-mediated transport of the AAA-ATPase Cdc48 protein at the MMD allows this disassembly factor to localize at the spindle midzone, thereby promoting spindle midzone dissolution. Our findings illustrate how a separate membrane compartment supports spindle disassembly in the closed mitosis of fission yeast.

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