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Nucleocapsid amino acids 211 to 254, in particular, tetrad glutamines, are essential for the interaction between the nucleocapsid and membrane proteins of SARS-associated coronavirus.

Authors
  • Fang, Xiaonan
  • Ye, Lin-Bai
  • Zhang, Yijuan
  • Li, Baozong
  • Li, Shanshan
  • Kong, Lingbao
  • Wang, Yuhua
  • Zheng, Hong
  • Wang, Wei
  • Wu, Zhenghui
Type
Published Article
Journal
Journal of microbiology (Seoul, Korea)
Publication Date
Oct 01, 2006
Volume
44
Issue
5
Pages
577–580
Identifiers
PMID: 17082754
Source
Medline
License
Unknown

Abstract

GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.

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