Affordable Access

Nucleic acid hybridization with RNA immobilized on filter paper.

Authors
  • Saxinger, W C
  • Ponnamperuma, C
  • Gillespie, D
Type
Published Article
Journal
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Publication Date
Oct 01, 1972
Volume
69
Issue
10
Pages
2975–2978
Identifiers
PMID: 4562748
Source
Medline
License
Unknown

Abstract

RNA has been immobilized in a manner suitable for use in molecular hybridization experiments with dissolved RNA or DNA by a nonaqueous solid-phase reaction with carbonyldiimidazole and RNA "dry coated" on cellulose or, preferably, on previously activated phosphocellulose filters. Immobilization of RNA does not appear to alter its chemical character or cause it to acquire affinity for unspecific RNA or DNA. The versatility and efficiency of this method make it potentially attractive for use in routine analytical or preparative hybridization experiments, among other applications.

Report this publication

Statistics

Seen <100 times