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Nuclear Accumulation of β-Catenin in Cancer Stem Cell Radioresistance and Stemness in Human Colon Cancer.

Authors
  • Tanaka, Hidekazu1, 2
  • Kawaguchi, Masaya1, 3
  • Shoda, Shinichi1, 4
  • Miyoshi, Toshiharu4
  • Iwasaki, Ryota5
  • Hyodo, Fuminori1, 6
  • Mori, Takashi7, 8
  • Hara, Akira3
  • Tomita, Hiroyuki9
  • Matsuo, Masayuki1
  • 1 Department of Radiology, Gifu University Graduate School of Medicine, Gifu, Japan. , (Japan)
  • 2 Department of Radiation Oncology, Yamaguchi University Graduate School of Medicine, Ube, Japan. , (Japan)
  • 3 Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan. , (Japan)
  • 4 Department of Radiology Service, Gifu University Hospital, Gifu, Japan. , (Japan)
  • 5 Animal Medical Center, Gifu University, Gifu, Japan. , (Japan)
  • 6 Department of Frontier Science for Imaging, Gifu University Graduate School of Medicine, Gifu, Japan. , (Japan)
  • 7 Department of Veterinary Clinical Oncology, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan. , (Japan)
  • 8 Center for Highly Advanced Integration of Nano and Life Science, Gifu University, Gifu, Japan. , (Japan)
  • 9 Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu, Japan [email protected] , (Japan)
Type
Published Article
Journal
Anticancer Research
Publisher
International Institute of Anticancer Research
Publication Date
Dec 01, 2019
Volume
39
Issue
12
Pages
6575–6583
Identifiers
DOI: 10.21873/anticanres.13873
PMID: 31810923
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The aim of this study was to examine whether the Wnt/β-catenin signal activation is a cause of radioresistance in colon cancer by assessing the β-catenin localization and its correlation with cancer stem cells (CSCs). The nuclear levels of β-catenin, the hallmark of Wnt activation, were analyzed in HCT116 and SW480 cells by immunohistochemistry, before and after irradiation. Further, we assessed CSC populations by staining for aldehyde dehydrogenase-1 (ALDH1) and CD44. β-catenin was localized predominantly in the nucleus and plasma membrane in SW480 and HCT116 cells, respectively. Compared to HCT116 cells, SW480 cells displayed higher Wnt activation. At 24 h after irradiation, most of the DSBs in SW480 cells were repaired, but were still present in HCT116 cells. Additionally, compared to HCT116 cells, a significantly higher proportion of SW480 cells were ALDH1- and CD44-positive. Colon cancers with nuclear β-catenin accumulation demonstrated greater radio-resistance with a higher number of CSCs. Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

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