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NPSN11 Is a Cell Plate-Associated SNARE Protein That Interacts with the Syntaxin KNOLLE1

Authors
  • Haiyan Zheng
  • Sebastian Y. Bednarek
  • Anton A. Sanderfoot
  • Jose Alonso
  • Joseph R. Ecker
  • Natasha V. Raikhel
Publisher
American Society of Plant Physiologists
Publication Date
Jun 01, 2002
Source
PMC
Keywords
Disciplines
  • Biology
  • Computer Science
License
Unknown

Abstract

NPSN11 Is a Cell Plate-Associated SNARE Protein That Interacts with the Syntaxin KNOLLE by Haiyan Zheng, Sebastian Y. Bednarek, Anton A. Sanderfoot, Jose Alonso, Joseph R. Ecker, and Natasha V. Raikhel Plantphysiol Volume 129(2):530-539 June 1, 2002 ©2002 by American Society of Plant Biologists NPSN group SNAREs are novel to the plant kingdom. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN11 and -12 have different tissue distribution patterns. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN11 T-DNA insertion mutant. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN11 and KNOLLE cofractionate on an Accudenz gradient. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN11 is localized at the cell plate during cytokinesis. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN11 interacts with the syntaxin KNOLLE. A, NPSN11 protein was immunoprecipitated from total membrane proteins prepared from 5-d-old Arabidopsis suspension-cultured cells. Zheng H et al. Plantphysiol 2002;129:530-539 ©2002 by American Society of Plant Biologists NPSN group SNAREs are novel to the plant kingdom. Representative SNARE protein sequences were acquired from GenBank (Arath, Arabidopsis; Sacce, budding yeast; Homsa, human; Musmu, mouse; see “Materials and Methods” for accession nos.) and aligned with the CLUSTALW algorithm. A phylogenetic tree was visualized with TreeView and was prepared for the figure with Adobe Photoshop. NPSN11 and -12 have different tissue distribution patterns. A, RT-PCR was performed with primers specific forNPSN11, NPSN12, NPSN13, andVTI12, using total RNA prepared from the roots of a 3-week-old liquid-cultured plants (lane 1), the leaves of the same liquid-cultured plants (lane 2), flowers from mature soil-grown plants (lane 3), expanding rosette lea

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