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Novel protein vaccine candidates against Group B streptococcal infection identified using alkaline phosphatase fusions.

Authors
  • Hughes, Martin J G
  • Wilson, Rebecca
  • Moore, Joanne C
  • Lane, Jonathan D
  • Dobson, Richard J
  • Muckett, Phillip
  • Younes, Zabin
  • Pribul, Philippa
  • Topping, Andrew
  • Feldman, Robert G
  • Santangelo, Joseph D
Type
Published Article
Journal
FEMS microbiology letters
Publication Date
May 28, 2003
Volume
222
Issue
2
Pages
263–271
Identifiers
PMID: 12770717
Source
Medline
License
Unknown

Abstract

Using an alkaline phosphatase-based genetic screening method, we identified a number of proteins that are potentially located on the outer surface of Group B streptococcus (Streptococcus agalactiae). In an enzyme-linked immunosorbent assay, antisera raised against two of the proteins, the streptococcal yutD homologue and a subunit of an ABC transporter, recognised clinically important serotypes of Group B streptococcus. In a neonatal rat model, purified IgG from the sera conferred significant levels of protection against a lethal challenge infection. The proteins identified show potential as protein subunit candidates for vaccines against Group B streptococcal disease in neonates.

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