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Novel planar and particle-based microarrays for point-of-care diagnostics

  • Svedberg, Gustav
Publication Date
Jan 01, 2019
DiVA - Academic Archive On-line
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Point-of-care assays are easy-to-use, portable and inexpensive tests that can be used to aid diagnostics by measuring levels of disease-specific molecules in settings where access to advanced laboratory equipment and trained personnel are limited, such as at the patient's bedside or in low resource parts of developing countries. In order to achieve high multiplexing capacities, such assays can be based on planar microarrays consisting of spots immobilized on a flat surface or on particle-based microarrays based on populations of encoded particles. The aim of the work presented in this thesis is to develop new point-of-care amenable planar and particle-based microarrays that allow for highly multiplexed assays while maintaining low sample-to-result times, complexity and instrumentation requirements. Paper I demonstrates the use graphically encoded particles for colorimetric detection of autoantibodies using a consumer-grade flatbed scanner. Four graphical characters on the surface of each particle allows for millions of codes and the use of gold nanoparticles as a detection label allows both the code and the signal intensity to be read out in a single image. Paper II describes a signal enhancement method that increases the sensitivity of gold nanoparticle detection on planar microarrays. Using this method, detection of allergen-specific IgE can be carried out using a consumer-grade flatbed scanner instead of a more expensive fluorescence scanner without sacrificing assay performance. Paper III demonstrates the use of an isothermal DNA amplification method for detection of adenoviral DNA on a paper-based microarray. Using an isothermal amplification method eliminates the need for a thermocycler, reducing the instrumentation required for such detection. Paper IV shows the use of solid-phase PCR to amplify bacterial DNA directly on the surface of particles. This strategy reduces assay time by eliminating the need for separate amplification and hybridisation steps. / <p>QC 20190221</p>

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