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A novel method for the extraction of outer membrane vesicles (OMVs) from Bordetella pertussis Tohama strain

Authors
  • Sekhavati, Mohammad1
  • Mobarez, Ashraf Mohabati1
  • Siadat, Seyed Davar2
  • Noofeli, Mojtaba3
  • 1 Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  • 2 Microbiology Research Center (MRC) Pasteur, Institute of Iran, Tehran, Iran
  • 3 Department of Human Bacterial Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Type
Published Article
Journal
Iranian Journal of Microbiology
Publisher
Tehran University of Medical Sciences
Publication Date
Feb 01, 2020
Volume
12
Issue
1
Pages
37–42
Identifiers
PMID: 32322378
PMCID: PMC7163034
Source
PubMed Central
Keywords
License
Green

Abstract

Background and Objectives: There are many pertussis outbreaks which is mainly due to the reduction in the immunity of acellular pertussis (aP) vaccines. Therefore, there is a crucial necessity to develop a new generation of pertussis vaccine. Preceding researches have shown that Bordetella pertussis outer membrane vesicles (OMVs) have appropriate specifications, making them a suitable vaccine candidate against pertussis. Materials and Methods: The OMVs were separated by a new serial ultra centrifugation technique. Transmission electron microscopy (TEM) examination, SDS-PAGE, Western blotting and ELISA assay were used to characterize the OMVs. Results: TEM studies showed the size of the extracted OMVs at 40–200 nm. The presence of pertussis toxin, filamentous hemagglutinin, and pertactin was verified using Western blot and ELISA assay. Conclusion: The presented technique is a simple and effective way to obtain OMVs from Bordetella pertussis. So it can be utilized as an appropriate procedure in the development of an OMV-based vaccine against pertussis.

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