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Novel method for the covalent immobilization of oligonucleotides via Diels-Alder bioconjugation.

Authors
  • Latham-Timmons, Hallie A
  • Wolter, Andreas
  • Roach, J Shawn
  • Giare, Rubina
  • Leuck, Michael
Type
Published Article
Journal
Nucleosides, nucleotides & nucleic acids
Publication Date
Jan 01, 2003
Volume
22
Issue
5-8
Pages
1495–1497
Identifiers
PMID: 14565451
Source
Medline
License
Unknown

Abstract

The synthesis of cyclohexadiene and maleimide derivatives and their use for the functionalization of oligonucleotides and the coating of glass surfaces is reported. A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented.

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