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A novel ladder-like lectin relates to sites of mucosal immunity in Atlantic halibut (Hippoglossus hippoglossus L.).

Authors
  • Magnadottir, Bergljot1
  • Gudmundsdottir, Sigridur2
  • Lange, Sigrun3
  • 1 Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112, Reykjavik, Iceland. Electronic address: [email protected] , (Iceland)
  • 2 Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112, Reykjavik, Iceland. Electronic address: [email protected] , (Iceland)
  • 3 Tissue Architecture and Regeneration Research Group, School of Life Sciences, University of Westminster, London, W1W 6UW, UK. Electronic address: [email protected]
Type
Published Article
Journal
Fish & Shellfish Immunology
Publisher
Elsevier
Publication Date
Dec 22, 2018
Volume
87
Pages
9–12
Identifiers
DOI: 10.1016/j.fsi.2018.12.034
PMID: 30584906
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A novel 27 kDa ladder-lectin-like protein, showing a multimeric structure under non-reducing conditions, was isolated from halibut serum by binding to N-acetyl glucosamine. Mass-spectrometry analysis did not show significant homology with known proteins. Specific antibodies were produced and used in immunohistochemistry on tissue sections of early halibut ontogeny from 119 until 1050 °d post hatching. A strong positive response was detected in the mucosal cells of the skin, gills and gut, indicating a role in the mucosal immune defence at these sites. Further immunopositivity was detected in liver, myeloma of kidney and the brain at different developmental stages but predominant expression was found in mucosal surfaces at later stages of development tested (1050 °d). It is still uncertain whether this ladder-like lectin forms part of the complement pathway, as a lectin or ficolin, or if it belongs to galectins. A strong detection in mucosal surfaces on skin, gills and gut, show similar patterns of expression as both mucosal lectins and galectins in other fish. Detection in neuronal tissue may indicate putative roles in tissue remodelling of brain and in ongoing neurogenesis in the fish eye. Copyright © 2018 Elsevier Ltd. All rights reserved.

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