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A novel human ex-vivo burn model and the local cooling effect of a bacterial nanocellulose-based wound dressing.

Authors
  • Holzer, Judith C J1
  • Tiffner, Katrin2
  • Kainz, Sonja2
  • Reisenegger, Peter2
  • Bernardelli de Mattos, Ives3
  • Funk, Martin4
  • Lemarchand, Thomas5
  • Laaff, Helmut5
  • Bal, Ayse5
  • Birngruber, Thomas2
  • Kotzbeck, Petra6
  • Kamolz, Lars-Peter6
  • 1 Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, Austria; COREMED - Cooperative Centre for Regenerative Medicine, Joanneum Research Forschungsgesellschaft mbH, Graz, Austria. Electronic address: [email protected] , (Austria)
  • 2 HEALTH - Institute for Biomedicine and Health Sciences, Joanneum Research Forschungsgesellschaft mbH, Graz, Austria. , (Austria)
  • 3 Fraunhofer Institute for Silicate Research ISC, Translational Center Regenerative Therapies, Würzburg, Germany. , (Germany)
  • 4 QRSKIN GmbH, 97076 Würzburg, Germany. , (Germany)
  • 5 TPL Path Labs, 79111 Freiburg, Germany. , (Germany)
  • 6 Division of Plastic, Aesthetic and Reconstructive Surgery, Department of Surgery, Medical University of Graz, Austria; COREMED - Cooperative Centre for Regenerative Medicine, Joanneum Research Forschungsgesellschaft mbH, Graz, Austria. , (Austria)
Type
Published Article
Journal
Burns : journal of the International Society for Burn Injuries
Publication Date
Dec 01, 2020
Volume
46
Issue
8
Pages
1924–1932
Identifiers
DOI: 10.1016/j.burns.2020.06.024
PMID: 32660829
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Burn wound progression is a significant problem as burns initially thought to be superficial can actually become full thickness over time. Cooling is an efficient method to reduce burn wound conversion. However, if the cooling agent is below room temperature, depending on the wound size the patient is at risk of hypothermia. Additionally, tissue perfusion is reduced leading to an aggravation of burn wound progression. We investigated if wound dressings based on non-pre-cooled bacterial nanocellulose (BNC) with a high water content cool a burn just by evaporation and reduce the intradermal damages in the skin. In a human ex-vivo model, skin explants underwent contact burns using a 100 °C hot steel block. The burned areas were divided into two groups of which one was cooled with a BNC-based wound dressing. Intradermal temperature probes measured temperature in cooled and uncooled burn sites over 24 h. For histological assessments of the burned areas biopsies were taken at different time points. High mobility group box-1 (HMBG1) staining served as marker for cell vitality and necrosis in the different skin layers. Intradermal temperature measurement showed that application of the BNC-based wound dressing reduced temperature significantly in burned skin. This cooling effect resulted in a maximum temperature difference of 6.4 ± 1.9 °C and a significant mean reduction of the area under the curve in the first hour after burn of 62% (p < 0.0001). The histological results showed less necrosis and less dermal-epidermal separation in the cooled areas. The HMGB1 staining revealed more vital cells in the cooled group than in the uncooled group. Based on our results, BNC-based wound dressings cool a burn. Intradermal temperature as well as thermal damage of the tissue was reduced. The tested BNC-based wound dressing can be used without pre-cooling to cool a burn as well as to reduce the burn BNC-based wound progression through its evaporation cooling effect. Copyright © 2020 Elsevier Ltd and ISBI. All rights reserved.

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