A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays.
- Authors
- Type
- Published Article
- Journal
- Review of Scientific Instruments
- Publisher
- American Institute of Physics
- Volume
- 79
- Issue
- 1
- Identifiers
- DOI: 10.1063/1.2830286
- Source
- Kit Lam Lab
- License
- Unknown
Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.