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A novel dimorphic microsporidian Ameson iseebi sp. nov. infecting muscle of the Japanese spiny lobster, Panulirus japonicus, in western Japan.

Authors
  • Itoh, Naoki1
  • Kuboyama, Azusa2
  • Freeman, Mark A3
  • Katata, Masahide4
  • Yamakawa, Takashi5
  • Yoshinaga, Tomoyoshi2
  • 1 Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 1138657, Japan. Electronic address: [email protected] , (Japan)
  • 2 Laboratory of Fish Diseases, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 1138657, Japan. , (Japan)
  • 3 Ross University, School of Veterinary Medicine, PO Box 334, Basseterre, Saint Kitts and Nevis. , (St. Kitts & Nevis)
  • 4 Wakayama Fisheries Experiment Station, Kushimoto, Wakayama 6493503, Japan. , (Japan)
  • 5 Laboratory of Fish Resources, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 1138657, Japan. , (Japan)
Type
Published Article
Journal
Journal of Invertebrate Pathology
Publisher
Elsevier
Publication Date
Oct 01, 2020
Volume
176
Pages
107472–107472
Identifiers
DOI: 10.1016/j.jip.2020.107472
PMID: 32926898
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Japanese spiny lobsters (Panulirus japonicus) exhibiting white opaque abdominal muscle were found in Mie and Wakayama prefectures, in mid-Western Japan. Microscopically, two types of microsporidian spores, ovoid and rod-shaped, were observed infecting the muscle. Histologically, both types of spore were detected inside myofibers of the abdomen, appendages, and cardiac muscles and were often both observed in a single myofiber simultaneously. Transmission electron microscopy revealed that ovoid spores have villous projections on the surface, and that ovoid and rod-shaped spores have a polar filament with 12 coils and 6 to 8 coils respectively. Merogonic and sporogonic stages were observed around ovoid spores, but rarely around rod-shaped spores. The small subunit ribosomal DNA sequences obtained from both spore types were identical to each other, indicating that this microsporidian exhibits a clear spore dimorphism. Phylogenetic analysis based on the rDNA sequences indicates that this microsporidian is part of a clade consisting of the genera Ameson and Nadelspora, with the most closely related species being A. herrnkindi found in the Caribbean spiny lobster P. argus. Based on ultrastructural features, molecular phylogenetic data, host type and geographical differences among known species in these genera, the species found in whitened abdominal muscles of the Japanese spiny lobster is described as Ameson iseebi sp. nov. Copyright © 2020 Elsevier Inc. All rights reserved.

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