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A novel anti-human ICOSL monoclonal antibody that enhances IgG production of B cells.

Authors
  • Chen, Jie
  • Wang, Fengming
  • Cai, Qiuping
  • Shen, Shuang
  • Chen, Youguo
  • Hao, Chao
  • Sun, Jing
Type
Published Article
Journal
Monoclonal antibodies in immunodiagnosis and immunotherapy
Publication Date
Apr 01, 2013
Volume
32
Issue
2
Pages
125–131
Identifiers
DOI: 10.1089/mab.2012.0121
PMID: 23607348
Source
Medline
License
Unknown

Abstract

ICOSL, a newly identified member of the B7 superfamily, plays a major role in immune responses. In this study, a functional anti-human ICOSL monoclonal antibody (MAb) 3B3 was obtained and characterized by means of flow cytometry, Western blot, and competition assay. This MAb could specifically recognize a distinct epitope of the ICOSL molecule. As a functional antibody, MAb 3B3 could inhibit the proliferation of T lymphocytes stimulated by ICOSL-L929 transfectants. Furthermore, it could enhance IgG production of PWM-driven B cells. The results indicate that the ICOS-ICOSL signal is critically involved in specific humoral immunity.

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