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Northern blotting analysis.

Authors
  • Josefsen, Knud
  • Nielsen, Henrik
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2011
Volume
703
Pages
87–105
Identifiers
DOI: 10.1007/978-1-59745-248-9_7
PMID: 21125485
Source
Medline
License
Unknown

Abstract

Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.

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