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A normobaric hypoxia facility for preparing polycythemic mice for assay of erythropoietin.

Authors
  • Hancher, C W
  • Smith, L H
Type
Published Article
Journal
Laboratory animal science
Publication Date
Feb 01, 1975
Volume
25
Issue
1
Pages
39–44
Identifiers
PMID: 1121163
Source
Medline
License
Unknown

Abstract

A normobaric hypoxia chamber was designed in which large numbers of mice (up to 250) were made polycythemic, after which they were used in an erythropoietin assay. The hypoxic atmosphere was composed of air diluted with N2 gas generated from liquid N2. Continuous exposure of mice to 8% O2 for 11 da resulted in a polycythemia of sufficient magnitude and duration to provide appropriate erythropoietic conditions for the erythropoietin assay. The chamber, which has been in almost continuous operation for 3 yr, requires little attention and maintenance and has proved to be reliable, safe, and economical.

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