Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells.
- Authors
- Publication Date
- Apr 19, 2019
- Source
- Apollo - University of Cambridge Repository
- Keywords
- Language
- English
- License
- Unknown
- External links
Abstract
Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.