Affordable Access

deepdyve-link
Publisher Website

Nonylphenol diethoxylate inhibits apoptosis induced in PC12 cells.

Authors
  • Liu, Chuang1
  • Sun, Yongkun2, 3
  • Song, Yutong1
  • Saito, Takeshi4
  • Kurasaki, Masaaki5, 6
  • 1 Graduate School of Environmental Science, Hokkaido University, Sapporo, 060-0810, Japan. , (Japan)
  • 2 Department of Basic Medicine, Xinxiang Medical University, Xinxiang, Henan, 453-003, China. , (China)
  • 3 Faculty of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan. , (Japan)
  • 4 Faculty of Health Sciences, Hokkaido University, Sapporo, 060-0812, Japan. , (Japan)
  • 5 Graduate School of Environmental Science, Hokkaido University, Sapporo, 060-0810, Japan. [email protected] , (Japan)
  • 6 Faculty of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan. [email protected] , (Japan)
Type
Published Article
Journal
Environmental toxicology
Publication Date
Nov 01, 2016
Volume
31
Issue
11
Pages
1389–1398
Identifiers
DOI: 10.1002/tox.22144
PMID: 25847295
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nonylphenol and short-chain nonylphenol ethoxylates such as NP2 EO are present in aquatic environment as wastewater contaminants, and their toxic effects on aquatic species have been reported. Apoptosis has been shown to be induced by serum deprivation or copper treatment. To understand the toxicity of nonylphenol diethoxylate, we investigated the effects of NP2 EO on apoptosis induced by serum deprivation and copper by using PC12 cell system. Nonylphenol diethoxylate itself showed no toxicity and recovered cell viability from apoptosis. In addition, nonylphenol diethoxylate decreased DNA fragmentation caused by apoptosis in PC12 cells. This phenomenon was confirmed after treating apoptotic PC12 cells with nonylphenol diethoxylate, whereas the cytochrome c release into the cytosol decreased as compared to that in apoptotic cells not treated with nonylphenol diethoxylates. Furthermore, Bax contents in apoptotic cells were reduced after exposure to nonylphenol diethoxylate. Thus, nonylphenol diethoxylate has the opposite effect on apoptosis in PC12 cells compared to nonylphenol, which enhances apoptosis induced by serum deprivation. The difference in structure of the two compounds is hypothesized to be responsible for this phenomenon. These results indicated that nonylphenol diethoxylate has capability to affect cell differentiation and development and has potentially harmful effect on organisms because of its unexpected impact on apoptosis. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1389-1398, 2016. © 2015 Wiley Periodicals, Inc.

Report this publication

Statistics

Seen <100 times