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Nonspecific reaction of a thiol: protein disulfide oxidoreductase with the disulfide bonds of insulin.

Authors
  • Pace, M
  • Pietta, P G
  • Fiorino, A
  • Pocaterra, E
  • Dixon, J E
Type
Published Article
Journal
Experientia
Publication Date
Oct 15, 1985
Volume
41
Issue
10
Pages
1332–1335
Identifiers
PMID: 3899711
Source
Medline
License
Unknown

Abstract

A thiol: protein disulfide oxidoreductase from bovine liver was isolated after separation from protein disulfide isomerase. The enzyme, after activation (reduction) with glutathione, was reacted with stoichiometric amounts of insulin and the sulfhydryl groups of the partially reduced hormone were labeled with iodo (l-14C)acetamide. After separation of the insulin chains, the radioactivity was found in both the peptides, with a ratio A-chain/B-chain equal to 2/1.

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