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Nonspecific reaction of a thiol: protein disulfide oxidoreductase with the disulfide bonds of insulin.

Authors
Type
Published Article
Journal
Experientia
0014-4754
Publication Date
Volume
41
Issue
10
Pages
1332–1335
Identifiers
PMID: 3899711
Source
Medline

Abstract

A thiol: protein disulfide oxidoreductase from bovine liver was isolated after separation from protein disulfide isomerase. The enzyme, after activation (reduction) with glutathione, was reacted with stoichiometric amounts of insulin and the sulfhydryl groups of the partially reduced hormone were labeled with iodo (l-14C)acetamide. After separation of the insulin chains, the radioactivity was found in both the peptides, with a ratio A-chain/B-chain equal to 2/1.

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