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Nonradioactive polynucleotide gene probe assay for the detection of three virulence toxin genes in diarrhoeal stools.

Authors
Type
Published Article
Journal
The Indian Journal of Medical Research
0971-5916
Publisher
Medknow Publications
Publication Date
Volume
95
Pages
121–124
Identifiers
PMID: 1506060
Source
Medline

Abstract

The present study describes a nonisotopic DNA-DNA hybridization assay for the detection of enterotoxigenic Escherichia coli (ETEC) in which the probes were labelled with the hapten molecule digoxigenin and after hybridization, DNA hybrids were detected by antidigoxigenin alkaline phosphatase conjugate. A blinded study carried out on a battery of enterotoxigenic and nonenterotoxigenic Esch. coli by dig-probe hybridization assay were compared with the results of a radiolabelled toxin gene probe hybridization assay (performed earlier). The three digoxigenin labelled probes gave a 100 per cent specificity and sensitivities of 95.45, 100 and 100 per cent for LT, STh and STp respectively. These results were comparable to those with the radioactive probes.

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