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Non-stationary 13C metabolic flux analysis of Chinese hamster ovary cells in batch culture using extracellular labeling highlights metabolic reversibility and compartmentation.

Authors
Type
Published Article
Journal
BMC Systems Biology
1752-0509
Publisher
Springer (Biomed Central Ltd.)
Publication Date
Volume
8
Pages
50–50
Identifiers
DOI: 10.1186/1752-0509-8-50
PMID: 24773761
Source
Medline
License
Unknown

Abstract

The CHO-K1 central metabolism in controlled batch culture proves to be robust. It has the main purpose to ensure fast growth on a mixture of substrates and also to mitigate oxidative stress. It achieves this by using compartmentation to control NADPH and NADH availability and by simultaneous synthesis and catabolism of amino acids.

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