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Non-selective lymphocyte isolation from human blood by nylon wool filtration and density centrifugation.

Authors
  • De Pauw, B E
  • Wessels, J M
  • Geestman, E J
  • Smeulders, J B
  • Wagener, D J
  • Haanen, C
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Jan 01, 1979
Volume
25
Issue
3
Pages
291–295
Identifiers
PMID: 310854
Source
Medline
License
Unknown

Abstract

A new method was employed to isolate lymphocytes from human peripheral blood. Continuous flow filtration through a nylon wool filter, at a flow rate of 1.4 ml/min, produced a lymphocyte yield of 90.5% and a purity of 96% without any shift in the B-T cell ratio. Ficoll-Isopaque with a specific gravity of 1.085 g/ml instead of 1.077 g/ml could be used to remove the erythrocytes. An overall recovery, including defibrination, filtration, Ficoll-Isopaque centrifugation and washing step, of 74.5% was achieved.

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