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Nitroreductase reactions of the NADPH: adrenodoxin reductase and the adrenodoxin complex.

Authors
  • Marcinkeviciene, J
  • Cenas, N
  • Kulys, J
  • Usanov, S A
  • Sukhova, N M
  • Selezneva, I S
  • Gryazev, V F
Type
Published Article
Journal
Biomedica biochimica acta
Publication Date
Jan 01, 1990
Volume
49
Issue
4
Pages
167–172
Identifiers
PMID: 2403337
Source
Medline
License
Unknown

Abstract

NADPH: adrenodoxin reductase (E.C. 1.18.1.2) and its complex with adrenodoxin catalyze the aerobic oxidation of NADPH by a number of substituted 2-nitrofurans, 5-nitroimidazoles and p-derivatives of nitrobenzene. The nitrocompounds are reduced via an initial single-electron transfer. Under anaerobic conditions nitrofurans are reduced to the corresponding amines. The rate constants of adrenodoxin oxidation by nitrocompounds vary from 4 x 10(5) to 3 x 10(2) M-1 s-1. A linear correlation between the rate constant logarithm and the single-electron reduction potential at pH 7.0 (E7(1)) of nitrocompounds was observed. The relation between the reactivity and the polarographic half-wave potential (E7(1/2)) is distorted. The reactivity of adrenodoxin reductase is two orders of magnitude lower than that of adrenodoxin.

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