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Nitrogen as the major factor influencing gene expression in Yarrowia lipolytica

Authors
  • Hapeta, Piotr1
  • Kerkhoven, Eduard J.2, 3
  • Lazar, Zbigniew1
  • 1 Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, Chelmonskiego 37, 51-630 Wroclaw, Poland
  • 2 Department of Biology and Biological Engineering, Division of Systems and Synthetic Biology, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
  • 3 Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
Type
Published Article
Journal
Biotechnology Reports
Publisher
Elsevier
Publication Date
Aug 27, 2020
Volume
27
Identifiers
DOI: 10.1016/j.btre.2020.e00521
PMID: 32923379
PMCID: PMC7476234
Source
PubMed Central
Keywords
License
Unknown

Abstract

Yarrowia lipolytica is an important industrial microorganism used for the production of oleochemicals. The design of effective biotechnological processes with this cell factory requires an in-depth knowledge of its metabolism. Here we present a transcriptomic study of Y. lipolytica grown in the presence of glycerol and glucose, and mixture of both at different carbon to nitrogen ratios. It emerged that the transcriptomic landscape of Y. lipolytica is more sensitive to the nitrogen availability than to the utilized carbon source, as evidenced by more genes being differentially expressed in lower carbon to nitrogen ratio. Specifically, expression of hexokinase ( HXK1 ) is significantly susceptible to changes in nitrogen concentrations. High HXK1 expression in low nitrogen seems to impact other genes which are implicated in tricarboxylic acid cycle and erythritol biosynthesis. We further show that expression of HXK1 and two genes belonging to the sugar porter family might be controlled by GATA-like zinc-finger proteins.

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