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Nitric oxide inhibits the HIV-1 reverse transcriptase activity.

Authors
  • Persichini, T
  • Colasanti, M
  • Fraziano, M
  • Colizzi, V
  • Medana, C
  • Polticelli, F
  • Venturini, G
  • Ascenzi, P
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
May 19, 1999
Volume
258
Issue
3
Pages
624–627
Identifiers
PMID: 10329434
Source
Medline
License
Unknown

Abstract

Nitric oxide (NO) is a polypotent regulatory molecule involved in a variety of activities, such as the modulation of the catalytic activity of cysteine-containing enzymes. The present study reports the modulation of the HIV-1 reverse transcriptase activity by NO, released by the NO-donors 3, 3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18), (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (NOR-3), 3-morpholinosydnonimine (SIN-1), 4-(phenylsulfonyl)-3-((2-(dimethylamino) ethyl)thio)furoxan oxalate (SNO-102), and sodium nitroprusside (SNP). NO inhibits dose-dependently the HIV-1 reverse transcriptase activity, likely due to oxidation of Cys residue(s). Present results, representing a new insight into the modulation mechanism of the HIV-1 reverse transcriptase activity, may be relevant to develop new strategies for inhibition of HIV-1 replication.

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