Affordable Access

Nitrate reductase in Escherichia coli K-12: involvement of chlC, chlE, and chlG loci.

Authors
  • V Stewart
  • C H MacGregor
Publication Date
Aug 01, 1982
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

We examined the properties of mutants of E. coli which are defective with respect to nitrate reductase activity. chlE::Mu cts and chlG::Mu cts mutants were all chlorate resistant, and the strains that we examined all synthesized nitrate reductase apoenzyme. We concluded that the chlE and chlG loci, like the chlA, chlB, and chlD loci, are involved in the synthesis of insertion of molybdenum cofactor. We identified at least four distinct phenotypic classes of chlC::Tn10 mutants, all of which were fully or partially sensitive to chlorate. Two of these classes may represent lesions in the structural genes for nitrate reductase subunits A and C. Two other classes may be altered in the regulation of the expression of nitrate reductase or other anaerobic enzymes. We propose the mnemonic nar for naming individual genes within the chlC locus.

Report this publication

Statistics

Seen <100 times