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NFATc3 deficiency reduces the classical activation of adipose tissue macrophages

Authors
  • Hu, Li1
  • He, Fengli1
  • Huang, Melfeng1
  • Peng, Melhua1
  • Zhou, Zhiguang1
  • Liu, Feng1, 2
  • Dai, Yan-Shan1
  • 1 Department of Metabolism and Endocrinology, Metabolic Syndrome Research Center, Key Laboratory of Diabetes Immunology, Ministry of Education, National Clinical Research Center for Metabolic Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China , (China)
  • 2 Department of Pharmacology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
Type
Published Article
Journal
Journal of Molecular Endocrinology
Publisher
Bioscientifica
Publication Date
Oct 01, 2018
Volume
61
Issue
3
Pages
79–89
Identifiers
DOI: 10.1530/JME-18-0070
PMID: 30307161
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Nuclear factors of activated T cells (NFAT) c3 have a prominent role in the regulation of proinflammatory factors in immune cells. The classically activated M1 macrophages are key players in the initiation and maintenance of adipose tissue (AT) inflammation. The role of NFATc3 in obesity and AT inflammation is unknown. We set out to determine how deficiency of NFATc3 effected macrophage polarization, inflammation and insulin resistance in visceral AT of high-fat diet (HFD)-fed mice. Nfatc3−/− and WT mice were fed a HFD for 8–17 weeks. Epididymal white AT (eWAT) F4/80(+) cells were characterized by fluorescence-activated cell sorting and quantitative RT-PCR. Results showed that Nfatc3−/− mice developed HFD-induced obesity similar to WT mice, but insulin sensitivity and glucose tolerance were improved, and liver fat accumulation was reduced in Nfatc3−/− mice compared to WT control mice. Moreover, M1 macrophage content and proinflammatory factors were reduced, whereas the alternatively activated M2 macrophage content was increased in eWAT of HFD-fed Nfatc3−/− mice compared to that of WT mice. In addition, eWAT insulin signaling was improved in HFD-fed Nfatc3−/− mice. Importantly, after bone-marrow-derived macrophages had been isolated from Nfatc3−/− mice and cultured in vitro, treatment of these cells with interferon-γ and lipopolysaccharide resulted in reduction of M1 inflammatory markers, suggesting that NFATc3 promoted M1 polarization by a cell-autonomous mechanism. The results demonstrated that NFATc3 played an important role in M1 macrophage polarization, AT inflammation and insulin resistance in response to obesity through transcriptional activation of proinflammatory genes. © 2018 Society for Endocrinology

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