Affordable Access

deepdyve-link
Publisher Website

New Wistar Kyoto and spontaneously hypertensive rat transgenic models with ubiquitous expression of green fluorescent protein.

Authors
  • Garcia Diaz, Ana Isabel1
  • Moyon, Ben2
  • Coan, Philip M3
  • Alfazema, Neza3
  • Venda, Lara4
  • Woollard, Kevin5
  • Aitman, Tim6
  • 1 Division of Immunology and Inflammation, Imperial College London, London W2 1PG, UK MRC Clinical Sciences Centre and Department of Medicine, Imperial College London, London W12 0NN, UK.
  • 2 Embryonic Stem Cell and Transgenics Facility, MRC Clinical Sciences Centre, Imperial College London, London W12 0NN, UK.
  • 3 Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK.
  • 4 MRC Clinical Sciences Centre and Department of Medicine, Imperial College London, London W12 0NN, UK.
  • 5 Division of Immunology and Inflammation, Imperial College London, London W2 1PG, UK [email protected] [email protected]
  • 6 MRC Clinical Sciences Centre and Department of Medicine, Imperial College London, London W12 0NN, UK Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK [email protected] [email protected]
Type
Published Article
Journal
Disease Models & Mechanisms
Publisher
The Company of Biologists
Publication Date
Apr 01, 2016
Volume
9
Issue
4
Pages
463–471
Identifiers
DOI: 10.1242/dmm.024208
PMID: 26769799
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The Wistar Kyoto (WKY) rat and the spontaneously hypertensive (SHR) rat inbred strains are well-established models for human crescentic glomerulonephritis (CRGN) and metabolic syndrome, respectively. Novel transgenic (Tg) strains add research opportunities and increase scientific value to well-established rat models. We have created two novel Tg strains using Sleeping Beauty transposon germline transgenesis, ubiquitously expressing green fluorescent protein (GFP) under the rat elongation factor 1 alpha (EF1a) promoter on the WKY and SHR genetic backgrounds. The Sleeping Beauty system functioned with high transgenesis efficiency; 75% of new rats born after embryo microinjections were transgene positive. By ligation-mediated PCR, we located the genome integration sites, confirming no exonic disruption and defining a single or low copy number of the transgenes in the new WKY-GFP and SHR-GFP Tg lines. We report GFP-bright expression in embryos, tissues and organs in both lines and show preliminaryin vitroandin vivoimaging data that demonstrate the utility of the new GFP-expressing lines for adoptive transfer, transplantation and fate mapping studies of CRGN, metabolic syndrome and other traits for which these strains have been extensively studied over the past four decades. © 2016. Published by The Company of Biologists Ltd.

Report this publication

Statistics

Seen <100 times