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A new uranium bioremediation approach using radio-tolerant Deinococcus radiodurans biofilm

Authors
  • Manobala, T1
  • Shukla, Sudhir K2
  • Subba Rao, T2
  • Dharmendira Kumar, M1
  • 1 Anna University, Department of Applied Science and Technology, Chennai, 600 025, India , Chennai (India)
  • 2 BARC Facilities, Biofouling and Thermal Ecology Section, Water and Steam Chemistry Division, Kalpakkam, 603 102, India , Kalpakkam (India)
Type
Published Article
Journal
Journal of Biosciences
Publisher
Springer-Verlag
Publication Date
Sep 21, 2019
Volume
44
Issue
5
Identifiers
DOI: 10.1007/s12038-019-9942-y
Source
Springer Nature
Keywords
License
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Abstract

Deinococcus radiodurans is the most radiation-tolerant organism ever known. It has gained importance in recent years as a potential candidate for bioremediation of heavy metals, especially the radioactive type. This study investigates the efficiency of a recombinant D. radiodurans (DR1-bf+) strain with an ability to form biofilm for uranium remediation. The modified Arsenazo III dye method was used to estimate the uranium concentration. Uranyl nitrate aqueous solution was generated during the operation of nuclear fuel reprocessing. The D. radiodurans biofilm (DR1-bf+) grown in the presence of 20 mM Ca2+ showed remarkable ability of uranyl ion removal. DR1-bf+ (+Ca2+) biofilm removed ~75±2% of 1000 mg/L uranium within 30 min post-treatment from uranyl nitrate aqueous solution. Uranium removal rate was also found to be directly proportional to biofilm age. This study discusses the ability of D. radiodurans biofilm in uranium removal.

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