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A new technique for obtaining mitotic chromosome spreads from fishes in the field.

Authors
  • Blanco, D R
  • Bertollo, L A C
  • Lui, R L
  • Vicari, M R
  • Margarido, V P
  • Artoni, R F
  • Moreira-Filho, O
Type
Published Article
Journal
Journal of fish biology
Publication Date
Jul 01, 2012
Volume
81
Issue
1
Pages
351–357
Identifiers
DOI: 10.1111/j.1095-8649.2012.03325.x
PMID: 22747826
Source
Medline
License
Unknown

Abstract

This study presents an adaptation of current methodologies for preparing mitotic chromosomes from fishes, optimized for use in the field. The high-quality preparations obtained using this modified methodology is suitable for subsequent chromosomal analysis. Importantly, this method is particularly useful when specimen collection sites are far from research laboratories or when researchers are working with highly sensitive species that do not survive long outside of their natural habitats.

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