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New specific markers of human and mouse fibroblasts.

Authors
  • Esterre, P
  • Melin, M
  • Serrar, M
  • Grimaud, J A
Type
Published Article
Journal
Cellular and molecular biology
Publication Date
May 01, 1992
Volume
38
Issue
3
Pages
297–301
Identifiers
PMID: 1611660
Source
Medline
License
Unknown

Abstract

The expression of different lineage-specific markers was examined on tissue-derived fibroblast cultures in three species (mouse, rat and human), using indirect immunofluorescence or labelled streptavidin-biotin techniques. Smooth muscle cells and a human monocyte-like cell line (U 937) were used as controls. Five monoclonal antibodies against human fibroblast epitopes et two against mouse fibroblasts were selected during this screening on cell cultures. Three surface markers (Thy-1, 6-19 and 1B10) and, particularly, one (5B5) cytoplasmic marker of human fibroblasts were suitable for labeling frozen, but not paraffin-embedded, tissue sections. Unfortunately, the two mouse fibroblast markers appeared of difficult and limited interest for this histopathologic strategy.

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