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New Recombinant Phytase from Kosakoniasacchari: Characteristics and Biotechnological Potential

Authors
  • Gordeeva, T. L.1
  • Borshchevskaya, L. N.1
  • Kalinina, A. N.1
  • Bulushova, N. V.1
  • Syneoky, S. P.1
  • Voronin, S. P.2
  • Kashirskaya, M. D.2
  • 1 State Research Institute for Genetics and Selection of Industrial Microorganisms, Kurchatov Institute National Research Center (Kurchatov Institute NRC, GOSNIIGENETIKA), Moscow, 117545, Russia , Moscow (Russia)
  • 2 BIOAMIDE Closed Joint Stock Co., Saratov, 410033, Russia , Saratov (Russia)
Type
Published Article
Journal
Applied Biochemistry and Microbiology
Publisher
Pleiades Publishing
Publication Date
Dec 01, 2020
Volume
56
Issue
7
Pages
779–786
Identifiers
DOI: 10.1134/S0003683820070042
Source
Springer Nature
Keywords
License
Yellow

Abstract

AbstractA DNA sequence from Kosakonia sacchari that, according to automated computer analysis, is believed to correspond to the gene for histidine-acid phytase has been selected from the GenBank database. The sequence was optimized for codon composition, synthesized, cloned, and expressed in Pichia pastoris. The main characteristics of the purified recombinant enzyme were determined. It was established that a pH of 4.5 and a temperature of 50°C are optimal for phytase functioning. The specific activity, Michaelis constant (Km), and maximum reaction rate (Vmax) with phytate as a substrate were 1470 U/mg, 193 μM and 2167 μmol/(min mg), respectively. It was shown that the enzyme was characterized by a wide range of working pH levels. Therefore, due to its properties, this new recombinant phytase can be considered a high-potential enzyme for agrobiotechnology.

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