Affordable Access

New PEGs for peptide and protein modification, suitable for identification of the PEGylation site.

Authors
  • Veronese, F M
  • Saccà, B
  • Polverino de Laureto, P
  • Sergi, M
  • Caliceti, P
  • Schiavon, O
  • Orsolini, P
Type
Published Article
Journal
Bioconjugate chemistry
Publication Date
Jan 01, 2001
Volume
12
Issue
1
Pages
62–70
Identifiers
PMID: 11170367
Source
Medline
License
Unknown

Abstract

New PEG derivatives were studied for peptide and protein modification, based upon an amino acid arm, Met-Nle or Met-beta Ala, activated as succinimidyl ester. PEG-Met-Nle-OSu or PEG-Met-beta Ala-OSu react with amino groups in protein-yielding conjugates with stable amide bond. From these conjugates PEG may be removed by BrCN treatment, leaving Nle or beta Ala as reporter amino acid, at the site where PEG was bound. The conjugation of PEG and its removal by BrCN treatment was assessed on a partial sequence of glucagone and on lysozyme as model peptide or protein. Furthermore, insulin, a protein with three potential sites of PEGylation, was modified by PEG-Met-Nle, and the PEG isomers were separated by HPLC. After removal of PEG, as reported above, the sites of PEGylation were identified by characterization of the two insulin chains obtained after reduction and carboxymethylation. Mass spectrometry, amino acid analysis and Edman sequence, could reveal the position of the reporter norleucine that corresponds to the position of PEG binding.

Report this publication

Statistics

Seen <100 times