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A new method for permeabilization of the plasma membrane of cultured mammalian cells. II. Mechanism for potentiation of bleomycin cytotoxicity by vortex-stirring with high molecular weight polyacrylic acid.

Authors
  • Shimizu, N
  • Kawazoe, Y
Type
Published Article
Journal
Biological & pharmaceutical bulletin
Publication Date
Jun 01, 1996
Volume
19
Issue
6
Pages
805–808
Identifiers
PMID: 8799476
Source
Medline
License
Unknown

Abstract

As previously reported, the cytotoxicity of bleomycin (BLM) toward cultured mammalian cells was remarkably potentiated by vortex-stirring this agent with the cells for a few seconds in the presence of high molecular weight polyacrylic acid (PAA). This paper describes that the enhancement of BLM cytotoxicity was also achieved by a sequential BLM-treatment immediately after vortex-stirring cells with PAA only. It was suggested that this enhancement resulted from the transient permeabilization of the plasma membrane induced during vortex-stirring in the presence of PAA, leading to an increase in BLM incorporation. This mechanism was supported by the finding of an appreciable uptake by vortex-stirred cells of Lucifer Yellow, a known non-permeant material. This procedure, which did not affect any cell viability, might be a beneficial method for transient permeabilization of cultured mammalian cells to facilitate the internalization of non-permeant materials.

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