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A new colorimetric assay method for the detection of anti-hepatitis C virus antibody with high sensitivity.

Authors
  • Gao, Tao1
  • Chai, Wenxin
  • Shi, Liu
  • Shi, Hai
  • Sheng, Anzhi
  • Yang, Jie
  • Li, Genxi
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Nov 07, 2019
Volume
144
Issue
21
Pages
6365–6370
Identifiers
DOI: 10.1039/c9an01466g
PMID: 31566645
Source
Medline
Language
English
License
Unknown

Abstract

The early detection of low abundance anti-hepatitis C virus antibody (anti-HCV Ab) is critical for efficient diagnosis and treatment of HCV infection. In this work, a new colorimetric assay method has been proposed for the sensitive detection of anti-HCV Ab. In this method, the antibody-induced DNA strand displacement and the resulting rolling circle amplification (RCA) are integrated to generate a large amount of tandemly repeated G-quadruplex DNAzymes on the arm of the "Y"-shaped antibody. Consequently, oxidation of 3,3',5,5'-tetramethylbenzidine can be extensively catalyzed by the peroxidase-mimicking DNAzymes. Therefore, the readout signal can be greatly amplified. Further studies reveal that 0.998 pM anti-HCV Ab can be detected by this newly developed assay method. Moreover, the strategy proposed in this method can be adapted for the detection of other antibodies or bivalent targets.

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