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New Approaches for Genome Assembly and Scaffolding

Authors
  • Rice, Edward S.
  • Green, Richard E.
Type
Published Article
Journal
Annual Review of Animal Biosciences
Publisher
Annual Reviews
Publication Date
Feb 15, 2019
Volume
7
Pages
17–40
Identifiers
DOI: 10.1146/annurev-animal-020518-115344
Source
Annual Reviews
Keywords
License
Yellow

Abstract

Affordable, high-throughput DNA sequencing has accelerated the pace of genome assembly over the past decade. Genome assemblies from high-throughput, short-read sequencing, however, are often not as contiguous as the first generation of genome assemblies. Whereas early genome assembly projects were often aided by clone maps or other mapping data, many current assembly projects forego these scaffolding data and only assemble genomes into smaller segments. Recently, new technologies have been invented that allow chromosome-scale assembly at a lower cost and faster speed than traditional methods. Here, we give an overview of the problem of chromosome-scale assembly and traditional methods for tackling this problem. We then review new technologies for chromosome-scale assembly and recent genome projects that used these technologies to create highly contiguous genome assemblies at low cost.

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