New approaches to chromatographic purification of bovine dopamine-beta-hydroxylase.
Centre Bioengineering, Russian Academy of Sciences, Vavilova, Moscow.
- Published Article
Journal of Chromatography A
- Publication Date
Sep 08, 1995
The use of the traditional scheme for the isolation of bovine dopamine-beta-hydroxylase (bDBH) from bovine adrenal medulla resulted in active but not pure bDBH, containing about 50% of admixtures. Immobilized metal chelate affinity chromatography on agarose modified with iminodiacetic acid residues and charged with cobalt ions was applied in the final stage to obtain more than 90% pure and active bDBH. Final purification of bDBH using step elution with 0-0.5 M methyl-D-mannoside in buffer solution from concanavalin A-Sepharose was studied. The determination of bDBH in various samples was performed using size-exclusion chromatography.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/7496482