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A new approach to the synthesis of a protected 2-aminopurine derivative and its incorporation into oligodeoxynucleotides containing the Eco RI and Bam HI recognition sites.

Authors
  • McLaughlin, L W
  • Leong, T
  • Benseler, F
  • Piel, N
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jun 24, 1988
Volume
16
Issue
12
Pages
5631–5644
Identifiers
PMID: 2838824
Source
Medline
License
Unknown

Abstract

A protected 2-aminopurine nucleoside suitable for incorporation into oligodeoxynucleotides using phosphite triester chemical synthesis procedures has been prepared via oxidation of a purine hydrazino derivative with silver (I) oxide. Five oligodeoxynucleotides containing Eco RI and Bam HI recognition sites have been prepared such that, in the double stranded form, the 2-aminopurine base has either a complementary thymine or cytosine nucleobase. The helix character and thermodynamic parameters for helix formation have been examined.

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