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Never at rest: insights into the conformational dynamics of ion channels from cryo-electron microscopy.

Authors
  • Lau, Carus1, 2
  • Hunter, Mark J1
  • Stewart, Alastair1, 2
  • Perozo, Eduardo3
  • Vandenberg, Jamie I1, 2
  • 1 Victor Chang Cardiac Research Institute, Darlinghurst, NSW, 2010, Australia. , (Australia)
  • 2 St Vincent's Clinical School, University of NSW, Darlinghurst, NSW, 2010, Australia. , (Australia)
  • 3 Department of Biochemistry and Molecular Biology, University of Chicago, Chicago, IL, 60637, USA.
Type
Published Article
Journal
The Journal of Physiology
Publisher
Wiley (Blackwell Publishing)
Publication Date
Apr 01, 2018
Volume
596
Issue
7
Pages
1107–1119
Identifiers
DOI: 10.1113/JP274888
PMID: 29377132
Source
Medline
Keywords
License
Unknown

Abstract

The tightly regulated opening and closure of ion channels underlies the electrical signals that are vital for a wide range of physiological processes. Two decades ago the first atomic level view of ion channel structures led to a detailed understanding of ion selectivity and conduction. In recent years, spectacular developments in the field of cryo-electron microscopy have resulted in cryo-EM superseding crystallography as the technique of choice for determining near-atomic resolution structures of ion channels. Here, we will review the recent developments in cryo-EM and its specific application to the study of ion channel gating. We will highlight the advantages and disadvantages of the current technology and where the field is likely to head in the next few years.

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