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Neutrophils suppress γδ T-cell function.

Authors
  • Sabbione, Florencia
  • Gabelloni, María L
  • Ernst, Glenda
  • Gori, María S
  • Salamone, Gabriela
  • Oleastro, Matías
  • Trevani, Analía
  • Geffner, Jorge
  • Jancic, Carolina C
Type
Published Article
Journal
European Journal of Immunology
Publisher
Wiley
Publication Date
Mar 01, 2014
Volume
44
Issue
3
Pages
819–830
Identifiers
DOI: 10.1002/eji.201343664
PMID: 24271816
Source
Medline
Keywords
License
Unknown

Abstract

γδ T cells have been shown to stimulate the recruitment and activation of neutrophils through the release of a range of cytokines and chemokines. Here, we investigated the reverse relationship, showing that human neutrophils suppress the function of human blood γδ T cells. We show that the upregulation of CD25 and CD69 expression, the production of IFN-γ, and the proliferation of γδ T cells induced by (E)-1-hydroxy-2-methylbut-2-enyl 4-diphosphate are inhibited by neutrophils. Spontaneous activation of γδ T cells in culture is also suppressed by neutrophils. We show that inhibitors of prostaglandin E2 and arginase I do not exert any effect, although, in contrast, catalase prevents the suppression of γδ T cells induced by neutrophils, suggesting the participation of neutrophil-derived ROS. We also show that the ROS-generating system xanthine/xanthine oxidase suppresses γδ T cells in a similar fashion to neutrophils, while neutrophils from chronic granulomatous disease patients only weakly inhibit γδ T cells. Our results reveal a bi-directional cross-talk between γδ T cells and neutrophils: while γδ T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn suppress the activation of γδ T cells to contribute to the resolution of inflammation.

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