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Neutral polar methacrylate-based monoliths for normal phase nano-LC and CEC of polar species including N-glycans.

Authors
  • 1
  • 1 Department of Chemistry, Oklahoma State University, Stillwater, OK 74078-3071, USA.
Type
Published Article
Journal
Journal of Separation Science
1615-9314
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
32
Issue
1
Pages
10–20
Identifiers
DOI: 10.1002/jssc.200800546
PMID: 19058161
Source
Medline
License
Unknown

Abstract

Neutral diol methacrylate-based monoliths were developed for normal phase chromatography (NPC) and NP-CEC of polar compounds including N-glycans. Four different diol methacrylate-based monoliths were synthesized via the copolymerization of a functional monomer using either glyceryl monomethacrylate (GMM) or glycidyl methacrylate (GMA) and a crosslinker either ethylene dimethacrylate (EDMA) or trimethylolpropane trimethacrylate (TRIM). While the GMM-based monoliths yield in one reaction step polar diol methacrylate monoliths that are ready for use in NPC or NP-CEC, the GMA-based monoliths required a postmodification with hot sulfuric acid to convert the epoxy functions into diols before use in NPC or NP-CEC. All the four monoliths are neutral and void of fixed charges on their surfaces but yet exhibited relatively strong EOF in NP-CEC. The EOF is attributed to the adsorption of ions from the mobile phase thus forming the electric double layer necessary for producing a bulk mobile phase flow. Under the same in situ copolymerization conditions of GMM or GMA with either EDMA or TRIM, the GMM-EDMA monolith was the best choice in terms of retention, separation efficiency, EOF velocity in CEC and linear flow velocity in Nano-LC.

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